ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
8(4): 204-209, 2008

VEGETATIVE PROPAGATION OF DIFFERENT CYCLAMEN SPECIES VIA ADVENTITIOUS SHOOT FORMATION FROM SEEDLING TISSUE

Anika Nadja Sabine Prange1*, Margrethe Serek1, and Traud Winkelmann2

1 Gottfried Wilhelm Leibniz University of Hannover, Institute of Floriculture and Woody Plant Science, Floriculture Section, 2 Herrenhaeuser str., D-30419 Hannover, Germany, *Fax: 0049 511 762 2654, *E-mail: prange@zier.uni-hannover.de
2 University of Applied Sciences Weihenstephan and Research Station for Horticulture,
Am Staudengarten 8, D-85354 Freising, Germany


Abstract
While Cyclamen persicum Miller has gained economic importance as an important ornamental plant worldwide, only a few of the other nineteen species of the same genus have commercial relevance although having a lot of interesting and valuable traits. To examine these traits under in vitro culture and greenhouse conditions an efficient in vitro propagation method of different Cyclamen species was established. Sterile seedlings of Cyclamen mirabile Hildebrand, Cyclamen coum Miller, Cyclamen graecum Link and Cyclamen hederifolium Aiton divided in cotyledon, tuber and roots were used as a starting material. Shoot induction was obtained on half-strength Murashige and Skoog based medium containing 0.5 mg l-1 NAA and 1 mg l-1 BAP and on medium with 0.5 mg l-1 IAA, 1 mg l-1 BAP, 1 mg l-1 2iP and 1 mg l-1 kinetin as growth regulators. The most suitable tissues for shoot induction were tubers and/or cotyledons depending on the species. Less or no shoots were obtained from roots when used as starting tissue, but root formation was found in high frequency. There were pronounced genotypic differences in the reaction to the different media within one species. The medium with lower contents of cytokinins showed high formation of roots, possibly due to high endogenous auxin content in the tissue.

Key words: adventitious shoot induction, cyclamen, in vitro propagation, organogenesis, ornamental plant, regeneration



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