ISSN 1311-9109 Journal Content

International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy

Propagation of Ornamental Plants
8(3): 151-153, 2008


Antje Doil1*, Rui Zhang2, Annegret Schum3, Margrethe Serek2, and Traud Winkelmann1

1* University of Applied Sciences Weihenstephan and Research Station for Horticulture,
Am Staudengarten 8, D-85354 Freising, Germany, *Fax: + 49 8161 715106,
2 Gottfried Wilhelm Leibniz University of Hannover, Institute of Ornamental and Woody Plant Science, Floriculture Section, 2 Herrenhaeuser str.,
D-30419 Hannover, Germany
3 Federal Research Centre for Cultivated Plants - Julius Kuehn Institute, Institute of
Resistance Research and Stress Tolerance, Rudolf-Schick-Platz 3,
D-18190 Gross Luesewitz, Germany

The aim of the first set of experiments was to optimize adventitious shoot regeneration in Hydrangea macrophylla 'Nachtigall'. For this purpose leaf explants were cultured on Murashige and Skoog (MS) and B5-Gamborg (B5) media either with full or half-strength macro- and micronutrients, respectively. After eight weeks the highest regeneration rate and the highest number of regenerated shoots were observed on half-strength B5 medium. Consequently this medium was used in the subsequent experiment in which different cytokinins were compared. BAP, TDZ and meta-Topolin (m-Top) each in two concentrations were used. No shoot regeneration was observed on media supplemented with m-Top and only poor regeneration obtained on TDZ containing media. Using BAP resulted in regeneration rates between 29.4 and 80% and 2 to 3.1 shoots per regenerating explant. Another experiment was devoted to axillary shoot propagation. For this purpose, culture on solid medium, in temporary immersion system (TIS) and in permanent immersion system (PIS) was compared. The highest shoot production was obtained in PIS but the plant quality was very low due to hyperhydricity and yellowing. Plants in TIS showed a slightly lower propagation rate but were of excellent quality.

Key words: adventitious shoots, cytokinin, Hydrangea, plant quality, temporary immersion system

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