ISSN 1311-9109 Journal Content

International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy

Propagation of Ornamental Plants
8(3): 144-147, 2008


Katsuaki Ishii*, Yoshihisa Hosoi, and Emilio Maruyama

Forestry and Forest Products Research Institute, Department of Molecular
and Cell Biology, Matsunosato 1, Tsukuba, Ibaraki 305-8687, Japan,
*Fax: +81-298-873-1542, *E-mail:

The effects of CO2 enrichment on surviving and rooting of woody plant cuttings and tissue culture were evaluated. Cuttings of two flower cherries, Prunus incisa Thunb. and Prunus jamasakura Sieb. cv. Hiuchidani-kikuzakura, one endangered five needle pine, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, 7 nematode resistant clones of Pinus densiflora Sieb et. Zucc. and 3 nematode resistant clones of Pinus thunbergii Parl. were evaluated both in normal ambient and under 0.1 % CO2 enriched condition. Tissue cultured shoots of Pinus thunbergii were cultured in the rooting medium with different number of milliseal® (Millipore, filter pore size is 0.22 μm, and hole diameter on the lid of plant box is 5 mm). After 2 months of cutting, flower cherries were all dead in the normal CO2 condition, however surviving rate in the CO2 enriched atmosphere was 92% and 75% for Prunus incisa and Prunus jamasakura respectively. Rooting rate was 8% and 33% with each species respectively only with CO2 enriched condition. After 6 months of cutting in Pinus armandii var. amamiana with CO2 enrichment, rooting was observed in the 3 out of 18 branches of I-19 adult tree and in the one out of 12 branches of I-12 adult tree while no rooting appeared without CO2 enrichment treatment. Branches of Pistacia chinensis were rooted under carbon dioxide enriched condition. A marked enhanced rooting rate of in vitro cultured shoots of Pinus thubergii was observed on florialite® (Iwaki Co., cellulose + vermiculite medium) with sugar-free liquid half-strength White medium in plant box with milliseal® under CO2 enriched condition.

Key words: CO2, Pinus armandii, Pinus densiflora, Pinus thunbergii, Prunus incisa, Prunus jamasakura, Pistacia chinensis

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