ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
7(1): 29-36, 2007

IN VITRO STEM ELONGATION OF PAPHIOPEDILUM DELENATII GUILLAUMIN AND SHOOT REGENERATION VIA STEM NODE CULTURE

Duong Tan Nhut1*, Dang Thi Thu Thuy1, Nguyen Trinh Don1, Vu Quoc Luan1, Nguyen Thanh Hai1, Kiem Tran Thanh Van2, and Chendanda Chengappa Chinnappa3

1Dalat Institute of Biology, 116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam
*Tel.: 84-63-831056, *Fax.: 84-63-831028, *E-mail: duongtannhut@gmail.com
2Institute of Plant Biotechnology, University of Paris-Sud, UMR 0569, F- 91405, Orsay Cedex, FranceT
3Department of Biological Sciences, University of Calgary, 2500 University Drive N.W., Calgary T2N 1N4, Alberta, Canada


Abstract
A novel method for Paphiopedilum delenatii Guillaumin micropropagation through shoots regenerated from elongated-plantlet-derived nodal segments was developed. Elongation of P. delenatii stem was induced under low photosynthetically active radiation (PAR). The elongated stems were then cut into nodal segments and cultured on modified MS medium supplemented with different concentrations of plant growth regulators for investigating their ability of shoot regeneration. Shoots were first initiated on modifited MS medium supplemented with 2.0 mg l-1 BA after 15 days culture. However, these shoots were very small in size. Bigger shoots were obtained on medium supplemented with 1.5 mg l-1 zeatin or 1.5 mg l-1 TDZ with the regeneration rate of 58% and 75%, respectively. Healthy stem-node-derived plantlets were transferred onto a rooting medium before acclimatizing in the greenhouse. Elongation and stem node culture of P. delenatii will pave the way for further studies on the propagation of some other plants, especially recalcitrant species that have similar characteristics as Paphiopedilum.

Key words: fluorescent light, light-emitting diodes, Paphiopedilum delenatii Guillaumin, stem elongation, stem node culture





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