ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
7(1): 16-22, 2007

EFFECTS OF IN VITRO LEAF EXPLANTS AND LEAF SIZE ON DIRECT SHOOT REGENERATION OF GLOXINIA

Duong Tan Nhut*, Thai Huu Phu, Phan Xuan Huyen, and Dang Thi Thu Thuy

Department of Plant Biotechnology, Dalat Institute of Biology
116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam,
*Tel.: +84 91 8313045, *Fax: +84 63 831028, *E-mail: duongtannhut@gmail.com


Abstract
An efficient protocol for in vitro propagation of gloxinia has been established in this study. Adventitious shoot regeneration was compared among various leaf-derived explants cultured on MS medium supplemented with different concentrations of BA, TDZ and NAA for investigating optimal explants and size of leaves for obtaining efficient shoot regeneration. Different organogenesis programs including shoot regeneration, caulogenesis and rhizogenesis were firstly observed within 3 weeks of culture. A great number of vigorous shoots were directly obtained from in vitro intact leaves, leaf laminas and leaf segments. Results showed that leaf laminas when cut into 6 parts were optimal for in vitro multiplication with a great number of newly forming shoots (46.2 shoots/explant) obtained in medium supplemented with 1.0 mg l-1 BA and 0.2 mg l-1 NAA, giving rise to a 100% of shoot formation within 4 weeks. Shoots were vigorously rooted on medium containing 0.2 mg l-1 BA and 0.1 mg l-1 NAA after 4 weeks of culture, and plantlets were then successfully acclimatized in greenhouse. Results from this study reveal a potential procedure for plant production simply via leaf culture and promise an efficient approach for transformation technique of this valuable plant because of its capacity of direct regeneration from leaf explants.

Key words: culture, gloxinia, leaf, leaf laminas, leaf segments



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