ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(3): 158-163, 2005

A WOUNDING METHOD AND LIQUID CULTURE IN PAPHIOPEDILUM DELENATII PROPAGATION

Duong Tan Nhut¹*, Phan Thi Thuy Trang¹, Nguyen Hong Vu¹, Dang Thi Thu Thuy¹, Dinh Van Khiem¹, Nguyen Van Binh¹ and K. Tran Thanh Van²

¹Dalat Institute of Biology, 116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam, *Tel. +84-63-831056, *Fax. +84-63-831028, e-mail: duongtannhut@yahoo.com
² Institut de Biotechnologie des Plantes, Universite de Paris-Sud, UMR 0569, F-91405 Orsay Cedex, France.


Abstract
An innovative method for mass shoot propagation of Paphiopedilum delenatii ('Pink' slipper orchid), an endemic and endangered species of Vietnam, was established in this study. Nine-month-old seeds cultured on Knudson C medium was the optimal condition for in vitro germination. Seeds began swelling and became greenish in color after 30 to 40 days of culture. Then, rounded bodies, known as protocorms, were observed. Leaves and roots developed after 90-100 days of subculturing on the same medium. The combination of wounding and liquid culture was shown to be appropriate for highly frequent adventitious shoot formation. Shoot regeneration rate of 5.2 per wounded seedling could be obtained in MS liquid media supplemented with 1.0 mg l-1 TDZ via this method.

Key words: germination, liquid culture, Paphiopedilum delenatii, TDZ, wounding.



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