ISSN 1311-9109 Journal Content





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on Production and Establishment of Micropropagated Plants
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Propagation of Ornamental Plants
5(3): 152-157, 2005

THIDIAZURON INDUCES HIGH FREQUENCY SHOOT BUD FORMATION FROM BEGONIA PETIOLE TRANSVERSE THIN CELL LAYER CULTURE

Duong Tan Nhut¹*, Nguyen Thanh Hai¹, Phan Xuan Huyen¹, Doan Thi Quynh Huong¹, Nguyen Thi Thuy Hang¹ and Jaime A. Teixeira da Silva²

¹ Department of Plant Biotechnology, Dalat Institute of Biology 116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam, Tel.: +84 91 8313045, Fax: +84 63 831028, e-mail: duongtannhut@yahoo.com
² Faculty of Agriculture, Kagawa University, Miki-cho, Ikenobe, 2393, Kagawa-ken, 761-0795, Japan


Abstract
The effect of thidiazuron (TDZ) on shoot bud formation of Begonia spp. using transverse thin cell layer (tTCL) technology is presented in this paper. tTCLs were cut from Begonia petioles about 0.8-1.0 mm thick and cultured on half-strength MS medium supplemented with various concentrations of BA, TDZ, and different combinations of BA or TDZ and NAA. The highest shoot bud formation was obtained on medium with 0.2 mg l-1 TDZ and 0.2 mg l-1 NAA, giving rise to 100% shoot formation after 8 weeks. TDZ at low concentrations (0.1-0.2 mg l-1) was effective for caulogenesis from Begonia tTCLs.

Key words: Begonia, Thidiazuron, shoot bud formation, tTCL.



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