ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(3): 146-150, 2005

POSITIVE EFFECTS OF BACILLUS SPP. ON THE GROWTH OF CHRYSANTHEMUM SPP. IN VITRO AND EX VITRO

Duong Tan Nhut

Dalat Institute of Biology, 116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam, Tel. +84 63 831056, Fax. +84 63 831028,
e-mail: duongtannhut@yahoo.com or nhutduong@hotmail.com


Abstract
A positive effect of Bacillus spp. on the growth and development of Chrysanthemum spp. in vitro and ex vitro is presented for the first time. Bacillus spp. were positively confirmed by Gram and spore formation tests. Bacillus spp. affected the growth and development of Chrysanthemum spp. plantlets after 4 weeks of culture. Affected parameters included plant height, root number, and plant fresh weight. Chrysanthemum plantlets that were infected with Bacillus spp. had significantly higher values in these growth parameters than uninfected, control Chrysanthemum plantlets. Moreover, in vitro plantlets continued their vigorous growth in the greenhouse 4 weeks after they were transferred to the greenhouse. The height, fresh weight, and survival rate of Bacillus spp.-infected Chrysanthemum spp. plantlets grown ex vitro remained significantly higher than those of uninfected, control Chrysanthemum plantlets. In addition to these positive effects on plant growth, once present in culture media, Bacillus spp. restrict the development of many other pathogens.

Key words: Bacillus spp., Bacteria, Chrysanthemum spp., disease, endophyte.



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