Propagation of Ornamental Plants
5(2): 67-73, 2005
ARTIFICIAL SEEDS FOR PROPAGATION AND PRESERVATION OF CYMBIDIUM SPP.
Duong Tan Nhut¹*, Tran Ngoc Thuy Tien¹, Mai Thi Ngoc Huong¹, Nguyen Thi Thanh Hien¹, Phan Xuan Huyen¹, Vu Quoc Luan¹, and Jaime A. Teixeira da Silva²
¹Department of Plant Biotechnology, Dalat Institute of Biology, 116 Xo Viet Nghe Tinh, Da Lat, Lam Dong, Vietnam, *Tel. +84 91 8313045, *Fax. +84 63 831028,
²Kagawa University, Department of Horticulture, Ikenobe, 761-0795, Japan.
This research reports the production of artificial seeds (synseeds) through the encapsulation of protocorm-like bodies (PLBs) of Cymbidium spp. Three-months-old PLBs were encapsulated in a sodium alginate solution at different concentrations. Thereafter, synseeds were germinated on different in vitro culture systems. In addition to the investigation of direct germination of these synseeds - with an external protective coating made of chitosan solution - the ex vitro performance of the in vitro-derived plantlets on a non-sterilized fern root substrate was also tested. Survival ability of these synseeds cultured in vitro was 100% and the regeneration capacity was also high. The synseeds did not show a reduction in survival rate after storage in sucrose-free liquid medium for one year. Synseeds showed 35.5% survival rate and 45.8% shoot formation when directly transferred to non-sterilized substrate after coating with chitosan solution. The plantlets derived from these synseeds had a survival rate of 100% after 6 months in a greenhouse.
Key words: Cymbidium, regeneration, sodium alginate, survival rate, synseed.