ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
4(1): 53-57, 2004

STEM DEVELOPMENT OF AMELANCHIER LAMARCKII (F.G. SCHROEDER) IN VITRO AND ITS IMPORTANCE FOR IN VITRO ROOTING

Ina Pinker* and Thomas Wagner

*Humboldt University of Berlin, Institute of Horticultural Sciences, Albrecht-Thaer-Weg 1, D-14195 Berlin, Germany, Phone: ++49-30-31471163, Fax: ++49-30-31471160,
e-mail: ina.pinker@agrar.hu-berlin.de


Abstract
Stem development of shoot cultures from Amelanchier lamarckii on multiplication medium was investigated concerning shoot length, leaf number, stem diameter and xylem development after 4 to 7 weeks. Shoot length was classified in five different shoot length classes. Shoot length, leaf number, stem diameter as well as formation of secondary xylem increased from week 4 to week 7 on multiplication medium. For A. lamarckii each shoot length class was characterized by a certain leaf number and shoot diameter. Leaf number and shoot diameter has hardly changed in one shoot length class in dependence of subculture duration. From this point of view the shoot length can be used to classify different shoot qualities. The development of xylem, however, was dependent on the shoot length class and the subculture period, too. The root formation in shoots with a length from 5 to 30 mm was not different. In shoots smaller than 5 mm or longer than 30 mm, however, the root formation was significantly lower.

Key words: Amelanchier lamarckii, woody plants, micropropagation, shoot quality, xylem formation, rooting



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