Propagation of Ornamental Plants
4(1): 47-52, 2004
EFFECT OF BENZYLADENINE AND THIDIAZURON ON IN VITRO SHOOT FORMATION FROM COTYLEDONARY NODES OF TAMARINDUS INDICA LINN.
Town Mohammad Hussain¹ ², Thummala Chandrasekhar¹, Mohammad Arifullah¹ and Ghanta Rama Gopal¹
¹Department of Botany, Sri Venkateswara University, Tirupati-517 502, Andhra Pradesh, India, Fax: 0091-87722-50274,e-mail: firstname.lastname@example.org
²Institute of Botany Academia Sinica, Room no 422, Nankang, Taipei, Taiwan, ROC.
A protocol for micropropagation of highly economically important tree Tamarindus indica
is presented. Significantly more number (18.4) of shoots were induced from cotyledonary nodes derived from 15-days old aseptic seedlings on L2 medium (1979) with benzyladenine (BA) followed by thidiazuron (TDZ) supplement (sequential application). Culture of explants on BA or TDZ, BA + TDZ (combined application) or on TDZ followed by BA supplemented L2 medium yielded less number of shoots when compared to sequential use of BA followed by TDZ. Addition of GA3 (0.5 mg l-1
) had showed positive effect in increasing frequency of shoot regeneration and shoot number. Shoot regeneration on media with kinetin, isopentenyladenine (2iP) or zeatin was significantly lower when compared to sequential use of BA and TDZ.
Proliferating shoot culture was established by repeatedly subculturing original cotyledonary nodes on shoot multiplication medium (BA to TDZ) after each harvest of newly formed shoots. About 90.0% of the shoots developed roots following transfer to quarter-strength L2 medium with 1.0 mg l-1
indole-3-acetic acid. Eighty percent of the plantlets were successfully acclimatized and established in soil.
Abbreviations: AS - Adenine Sulphate, BA - Benzyladenine, IAA - Indole-3-acetic acid, GA3 - Gibberellic acid, IBA - Indole-3-butyric acid, 2iP - 2-isopentenyl adenine, NAA - α-Napthalene acetic acid, TDZ - Thidiazuron.
Key words: cotyledonary node, leguminous tree, micropropagation, Tamarindus indica