This study aimed to investigate the regrowth ability of Rosa × damascena ‘Trigintipetala’ synthetic seeds over a four-month storage period. Synthetic seeds were formed from shoot tips of in vitro grown Rosa × damascena ‘Trigintipetala’ using 3% sodium alginate in half-strength Murashige and Skoog (MS) nutrients solution as the gel matrix and 100 mM calcium chloride for complexation. Synthetic seed formation was achieved by dropping the sodium alginate/explant combination into a 100 mM calcium chloride aseptic solution for 25-30 min. Encapsulated shoot tips were transferred into aseptic Petri dishes containing 20 ml of 0.7% agar and stored at 4°C for four months. The regrowth ability of synthetic seeds into plantlets was assessed on MS medium containing 0.5 mg l^-1 6-benzylaminopurine (BAP) and 1.0 mg l^-1 gibberellic acid (GA₃). The highest regrowth rates of 93.3% and 86.7% were obtained following two and three months of cold storage, respectively. However, a low regrowth rate of 33.3% was observed after four months. The encapsulation procedure described here could significantly facilitate germplasm exchange and conservation programs for Rosa × damascena ‘Trigintipetala’.