Habenaria dentata (Sw.) Schltr. is a rare terrestrial orchid with high ornamental value, recently recorded in Korea. This study aimed to develop an efficient asymbiotic seed germination and regeneration protocol for the conservation and horticultural propagation of this species. Murashige and Skoog (MS) medium resulted in the highest seed germination rate (38.8%), significantly outperforming Vacin and Went (VW, 23.4%) and Hyponex (12.8%) media. Further optimization using MS medium supplemented with α-naphthaleneacetic acid (NAA; 0.5-4.0 µM) and activated charcoal (AC, 500-2000 mg l^-¹) revealed that a combination of 2.0 µM NAA and 500 mg l^-¹ AC supported the best morphogenetic response: 94.2% seed germination (18 days after culture), 89.9% protocorm formation (30 days), 85.0% shoot bud initiation (60 days), and 83.7% seedling development (140 days). All developmental stages were achieved on the same medium without subculturing or stage-specific transfers, highlighting a simplified and highly effective single-medium system. The synergistic effect of NAA and AC not only enhanced early germination but also promoted subsequent seedling development. The seedlings planted in sphagnum moss showed a 93% acclimatization rate.