A simple and efficient protocol was developed for in vitro regeneration via callus-mediated organogenesis of Ardisia silvestris Pitard. Leaf, nodal segments, and petiole explants from wild mature plants were used as initial materials for callus induction and plant regeneration. Effects of plant growth regulators, the types of explants on the formation of callus, and adventitious buds were studied. The research demonstrated that the optimal medium for callus induction from three type of explants was determined as Murashige and Skoog (MS) basal nutrients supplemented with 1.0 mg l^-1 Thidiazuron (TDZ) and solidified with 0.8% agar. This medium achieved a callus induction rate of > 80%. The MS medium supplemented with 1 mg l^-1 TDZ and 0.4 mg l^-1 indole-3-acetic acid (IAA) was most effective on shoot induction, with the highest shoot-induction rate and numbers of shoots on leaf explant (85% and 14.6, resp.). For rooting of shoots, MS medium supplemented with 1.5 mg l^-1 indole-3-butyric acid (IBA) showed the highest rooting regeneration rate (90%).