ISSN 1311-9109 Journal Content

International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy

Propagation of Ornamental Plants
19(1): 11-17, 2019


Fatih Tonguç1*, Mahmut Reis2, Muhammet Tonguç3, and Sercan Önder3

1 Isparta Applied Sciences University, Faculty of Forestry, 32260 Isparta, Turkey,
*Fax: + 90 246 211 39 48, *E-mail:
2 Kahramanmaraş Sutcu Imam University, Faculty of Forestry, 46110 Kahramanmaraş, Turkey
3 Isparta Applied Sciences University, Faculty of Agricultural Sciences and Technology, Department of Biotechnology, 32260 Isparta, Turkey

The present study was conducted to determine the morphological, biochemical, and germination characteristics of seeds of Hedysarum pogonocarpum Boiss., an endemic ornamental herbaceous plant in Turkey. Seeds were collected from three provenances (Kahramanmaraş, Adıyaman, and Malatya) and seed length, width, thickness, and weight of 1000 seed were estimated. Seed morphological characters showed significant differences among the provenances. Chemical composition (total soluble sugars, reducing sugars, carotenoids, xanthopyll, soluble protein, tocopherol, soluble phenolics, flavanoids, and oil content) were also examined and seven of the biochemical parameters showed significant differences among the provenances. Germination tests were conducted; seeds were immersed in hot water (50°C and 70°C) for 10, 20, and 30 min and stratified for 2, 4, and 6 weeks. Control seeds did not germinate both stratified (up to 6 weeks) or not. Hot water treatments were effective in breaking the dormancy. Provenances had also effect on the germination percentages. The highest germination rates were obtained from Kahramanmaraş provenance at 6 week stratified and soaked seeds at 50°C for 10 min (53.3%) and 20 min (49.3%). Two and four week stratification treatments yielded lower germination rates for the same treatments. Soaking in hot water at a higher temperature (70°C) yielded lower germination rates. According to the results of the study, it might be suggested that the seeds required 6 weeks or longer chilling time at 50°C and not more than 20 min soaking for the dormancy breaking and germination.

Key words: dormancy, oil content, protein, scarification, seed reserves, stratification

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