ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
18(1): 26-36, 2018

FLOWERING AND FLORAL DEVELOPMENT OF DENDROBIUM OFFICINALE KIMURA ET MIGO PLANTLETS IN VITRO UNDER DIFFERENT LIGHT SPECTRA

Tao Wen1, Liangqin Chen1, Weixuan Su1, Ruining Li1, Xuelei Jiao1, Xiaoying Liu1,
Zhigang Xu1*, and Zengxu Xiang2

1 College of Agronomy, Nanjing Agricultural University, Nanjing, 1 Weigang str., Xuanwu district, Nanjing, Jiangsu province, Jiangsu Province 210095, China, *Fax: + 86-25-8439-9709, *E-mail: xuzhigang@njau.edu.cn
2 College of Horticulture, Nanjing Agricultural University, Nanjing, 1 Weigang str., Xuanwu district, Nanjing, Jiangsu province, Jiangsu Province 210095, China



The effects of different light conditions on the flowering of Dendrobium officinale plantlets in vitro were determined. Single nodes of D. officinale (approx. 2.0 cm long with 2-3 leaves) were grown for 100 days under six different light conditions created by light emitting diodes. The six light conditions were as follows: blue and red at 660 nm or 630 nm (R660B and R630B), blue and green and red (R660BG and R630BG), and blue and yellow and red (R660BY and R630BY). Cultures grown under fluorescent lamps (FL) served as a control. The indices of growth, flowering, and floral development of D. officinale plantlets in vitro were investigated. The results showed that the 630-nm red light resulted in greater plant height and earlier flowering. Compared with 630-nm red light, 660-nm red light in the complex spectrum resulted in greater plantlet and flower dry mass, larger stem diameter, and peduncles, thicker leaves and petals, a longer blooming period, a greater number of open flowers, higher flowering percentage, increased pollen vigor, and higher carotenoid contents in petals. Green light added to the complex spectra of red and blue light resulted in greater plantlet dry mass, larger stem diameter, and thicker leaves. Yellow light promoted carotenoid accumulation in the petals. Red light at 660-nm promoted sugar transport from leaves to stems and stem tips, thereby promoting flowering.

Key words: flowering in vitro, floral development, LED



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