Propagation of Ornamental Plants
13(4): 154-159, 2013
IN VITRO CONSERVATION OF ENDANGERED ORCHID BULBOPHYLLUM AURICOMUM LINDL., THE ROYAL ORCHID OF MYANMAR
Myo Ma Ma Than
Centre of Advanced Study, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700 019 Kolkata, India, Fax: + 91-33-2461-4849,
In vitro conservation method of Bulbophyllum auricomum Lindl. has been developed in the present study. Minimum shoot length (7.68 ± 0.71 mm) and least root formation (at least 1 root/plantlet) were achieved when protocorm-derived shoots were cultured continuously on quarter-strength MS (Murashige and Skoog 1962) medium with 60 g l-1 sucrose for 12 months. In contrast, when the explants were maintained on full-strength MS nutrient medium supplemented with 30 g l-1 sucrose, the cultures grew faster, filled the culture vessel after 6 months of culture if not subcultured. To determine the growth and viability of prolong cultures, 20 randomly selected plantlets cultured on full-, half-, and quarter-strength MS medium with 30 or 60 g l-1 sucrose concentration were transferred to normal MS medium with 30 g l-1 sucrose. It was noted that all plantlets from different treatments were able to resume growth on MS basal medium after 3, 6 or 12 months of continuous culture on the same medium in the same culture vessel. There was no significant difference in survival percentage of shoots (80-100%) after culture for 3, 6, and 12 months on different strength of MS nutrient medium. While, quarter-strength MS medium with high sucrose concentration (60 g l-1) showed a retarding effect on growth of B. auricomum plants.
Key words: endangered species, growth retardation, nutrient medium, sucrose concentration