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Propagation of Ornamental Plants
13(3): 123-129, 2013
MICROPROPAGATION OF RHODODENDRON KEISKEI VAR. HYPOGLAUCUM SUTO & SUZUKI AND ASSESSMENT OF CLONAL FIDELITY
OF PLANTLETS BY RAPD
Iyyakkannu Sivanesan1 and Byoung Ryong Jeong1,2,3*
1 Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Korea 660-701 *Fax: +82-55-757-7542, *E-mail: brjeong@gnu.ac.kr
2 Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University, Jinju, Korea 660-701
3 Research Institute of Life Science, Gyeongsang National University, Jinju, Korea 660-701
An efficient reproducible protocol has been developed for in vitro propagation of Rhododendron keiskei var. hypoglaucum. Nodal explants were cultured on Anderson basal nutrients (AM) medium supplemented with different concentrations and combinations of plant growth regulators for axillary shoot proliferation. The highest percentage of shoot induction (96%) was achieved when nodal explants were cultured on AM medium supplemented with 2.0 mg l-1 2iP, 0.5 mg l-1 IAA, and 1.0 mg l-1 GA3 with a mean of 16 shoots per explant. Individual shoots, which were grown to about 2-3 cm long, were transferred onto a full or half strength AM medium with 0, 0.5, 1.0, 2.0 or 4.0 mg l-1 IBA or NAA for rooting. The highest percentage of rooting (100%), with maximum number of 7.6 roots per shoot and the greatest root length (4.4 cm) were obtained on the half-strength AM medium supplemented with 0.5 mg l-1 IBA. Random amplified polymorphic DNA analysis confirmed that the regenerated plantlets were genetically identical to their donor plant.
Key words: 2iP, Anderson medium, conservation, endangered species, in vitro propagation
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