Propagation of Ornamental Plants
10(3): 156-162, 2010
A COMPARATIVE STUDY OF IN VITRO REGENERATION OF SHOOTS IN DIFFERENT CULTIVARS OF GERBERA JAMESONII H. BOLUS EX HOOK. F.
Pratibha Misra*, Dhermendra Kumar Purshottam, Saema Siddiqui,
Madhu Bala Jain, and Dibya Dolridge Toppo
Plant Tissue Culture, National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow-226001(UP), India,
*Fax: + 91 522 2205836/2205839, *E-mail: email@example.com
Gerbera is the most popular ornamental plant of temperate climate but few cultivars grow in subtropical climate. For popularization of this crop in north Indian plains, like Lucknow, tissue culture is the only alternative method to begin with. A lot of work on tissue culture of gerbera has been done but the selection of suitable cultivars growing in hotter climates, their regeneration potential and the selection of proper explants to obtain maximum shoots in minimum period is the major concern of gerbera growers. In the present communication, responses of explants from different cultivars have been compared under in vitro conditions. The problem in the establishment of different cultivars due to phenolic contents could be overcome with the addition of 15 mg l-1 each of ascorbic acid and cysteine to the medium. The responses of different explants and the time required for initial regeneration of shoots has been studied in detail, where inflorescence and peduncle were observed as the most suitable explants for initial establishment of explants. The pattern of conversion of floral axis to vegetative axis and the development of adventitious shoots from different florets is presented. Organogenic cultures were obtained in Murashige and Skoog’s medium supplemented with 0.5 mg l-1 BA and 1.0 mg l-1 IAA. Regenerated shoots were rooted in plant growth regulator-free medium and after hardening grown under nethouse, where they flowered. It could be inferred from this investigation that gerbera cultivars ‘Dana Ellen’, ‘Salvador’ and ‘Rosaline’ are the best cultivars to grow in this climate, as compared to ‘Goliath’ and ‘Silvester’, whereas, ‘Sunway’ is not performing well under field conditions.
Key words: conversion of floral axis, cultivars, gerbera, inflorescence, plant growth regulators, tissue culture