Propagation of Ornamental Plants
10(3): 149-155, 2010
IN VITRO ADVENTITIOUS SHOOT ORGANOGENESIS AND PLANTLET REGENERATION FROM LEAF-DERIVED CALLUS OF
LAGERSTROEMIA SPECIOSA (L.) PERS.
Md Mahabubur Rahman1,2*, Muhammad Nurul Amin2,
Md Bablur Rahman2, and Rubaiyat Sharmin Sultana2
1Present address: Research Institute of Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan, *Fax: + 81-774-38-3682, *E-mail: firstname.lastname@example.org
2 Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh
High efficiency shoot regeneration was achieved through leaf-derived callus of Lagerstroemia speciosa on modified Murashige and Skoog medium (MMS; half strength macroelements, full strength microelements, and vitamins of MS medium) supplemented with plant growth regulators. The leaf-derived calluses on MMS medium fortified with 4.0 µM 2,4-D + 1.0 µM BAP + 568 µM ascorbic acid (AA) were regenerated maximum shoots on MMS + 5.0 µM BAP + 3.0 µM NAA + 10% coconut water + 568 µM AA. Shoot regeneration ability of the callus was investigated up to eight passages. The number of shoots per culture increased gradually up to 6th subculture, and more than 110 shoots were produced per leaf segment derived callus clump, but no remarkable changes were observed in the percentage of callus forming shoot and length of shoot throughout the subcultures. The root forming performance on MMS medium supplemented with 1.0 µM IBA was proved to be the best among three auxins studied.
Key words: antioxidant, callus culture, coconut water, Lagerstroemia speciosa, leaf explant Propagation of Ornamental Plants