In vitro culture of Adansonia digitata (baobab), an important multipurpose tree, was performed. A. digitata seeds showed the best response to surface sterilization and were aseptically germinated and cultured in vitro. A high concentration of BAP suppressed root development in germination in vitro. All explants produced flourishing callus growth in vitro except the root segment. Callus formation was higher in half-strength Quoirin and Lepoivre (½LP) medium than in half-strengh Murashige and Skoog or half-strength Gamborg’s B5 medium. The cotyledonary portion was the best explant for callus formation. Shoots were obtained from nodal segments of in vitro germinated A. digitata cultured on ½LP medium containing 10 µM BAP. A rooted plantlet was regenerated on half-strength woody plant medium containing 3.5 µM IBA and 0.32 µM NAA. A regenerated plantlet was obtained in vitro for the first time.