ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(4): 219-223, 2005

RAPID CLONAL PROPAGATION FROM NODAL EXPLANTS AND IN VITRO FLOWERING OF THREE ROSE CULTIVARS

Anuradha Mohapatra, Gyana Ranjan Rout*, and Premananda Das

Plant Tissue Culture Laboratory, Plant Biotechnology Division,
Regional Plant Resource Centre, Nayapalli, Bhubaneswar-751 015, Orissa, India,
*Fax: 0091-674-2550274, *E-mail: grrout@hotmail.com/grrout@rediffmail.com


Abstract
An efficient protocol was developed for in vitro clonal propagation of Rosa hybrida cvs. Cotton Tail, Jennifer and Pusa Gaurav from nodal culture. Multiple shoots were regenerated on MS medium supplemented with 0.5 mg l-1 6-benzylaminopurine (BAP), 20 mg l-1 adenine sulfate (Ads) and 0.1 mg l-1 indole-3-acetic acid (IAA), and 3% (w/v) sucrose. The number of shoots per explant varied depending on the plant growth regulator and the cultivar. Adding BAP, in combination with Ads and IAA, to the culture medium increased the frequency of production of multiple shoots in all three cultivars. In vitro flowering was achieved from microshoots grown on MS medium supplemented with 1.0 mg l-1 BAP, 50 mg l-1 Ads, and 0.01 mg l-1 IAA within 12 weeks of culture. The frequency of in vitro flowering was over 50% depending upon the cultivar. Highest rooting was achieved when shoots were transferred onto half-strength MS medium supplemented with 0.1 mg l-1 IAA, 0.1 mg l-1 2,4-D, and 2% (w/v) sucrose. Micropropagules were hardened in the greenhouse, and subsequently established in the open field.

Key words: hybrid rose, nodal explants, plant growth regulator, tissue culture.



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