ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(4): 199-204, 2005

PROPAGATION OF JAPANESE RED PINE (PINUS DENSIFLORA ZIEB. ET ZUCC.) VIA SOMATIC EMBRYOGENESIS

Emilio Maruyama*, Yoshihisa Hosoi, and Katsuaki Ishii

Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norinkenkyudanchi-Nai, Ibaraki, 305-8687 Japan
*Fax: +81-29-873-1542, *E-mail: tsumaruy@ffpri.affrc.go.jp


Abstract
Somatic embryogenesis in Pinus densiflora was initiated from megagametophytes containing immature zygotic embryos on media both with and without plant growth regulators (PGR). Embryogenic cultures were maintained and proliferated by 2- to 3-week interval subcultures in medium supplemented with 2,4-dichlorophenoxyacetic acid and N6-benzyladenine. An average of more than 700 somatic embryos per Petri dish was obtained after about 8 weeks of culture on maturation medium containing 60 g l–1 maltose, 75 g l–1 polyethylene glycol, 2 g l–1 activated charcoal, and 100 µM abscisic acid. Somatic embryos developed into plantlets on PGR-free medium with a frequency of 30%.

Key words: Embryo maturation, Japanese red pine, Pinus densiflora, propagation, somatic embryogenesis.



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