ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(4): 173-178, 2005

IN VITROPROPAGATION OF THREE ENDANGERED SPECIES IN JAPANESE FORESTS

Katsuaki Ishii¹*, Emilio Maruyama¹ Yoshihisa Hosoi¹, and Seiichi Kanetani²

¹1Transformation Laboratory, Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute, P. O. Box 16, Tsukuba Norinkenkyudanchi-nai, Ibaraki-ken, 305-8687, Japan,*Fax: +81-29-873-1542
*E-mail: katsuaki@ffpri.affrc.go.jp
²Ecological Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute, P. O. Box 16, Tsukuba Norinkenkyudanchi-nai, Ibaraki 305-8687, Japan


Abstract
In vitro propagation techniques were developed for three endangered plants found in Japanese forests, namely Cypripedium guttatum, Glaucidium palmatum, and Pinus armandii var. amamiana. Cypripedium guttatum was propagated via protocorm induction of immature seeds on a third-strength MS medium containing 1 μM BAP. Glaucidium palmatum was propagated via somatic embryogenesis from immature seeds on a half-strength MS medium containing 2.22 μM BAP. Pinus armandii var. amamiana was propagated via both organ culture of mature embryos on a half-strength DCR medium containing 0.4-2 μM BAP and somatic embryogenesis of immature or mature embryos on an ammonium-free MS or a half-strength EM medium. In vitro culture may help supply stock plants for the rehabilitation of damaged populations of herbal plants, or provide rootstocks for other propagation methods (e.g., grafting and producing seeds in seed orchards) in endangered trees.

Key words: Cypripedium guttatum, Glaucidium palmatum, Pinus armandii var. amamiana, somatic embryogenesis





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