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Propagation of Ornamental Plants
5(2): 78-83, 2005
CRYOPRESERVATION FOR GENE CONSERVATION OF ACER MONO MAX
Young-Goo Park¹*, Gi-Sok Kwon² and David Tay³
¹1Dept. of Forestry, Kyunpook National University, Deagu 702-701, Republic of Korea, *Fax: 82-53-950-6708, *e-mail: ygpark@mail.knu.ac.kr
²R&D Institute Tongyang Moolsan Co., Ltd.,Yangji-Myeon, Yongin-Si, Gyeonggi-Do 449-825, Republic of Korea
³Ornamental Plant Germplasm Center, The Ohio State University, 670 Vernon Tharp Street, Columbus, OH 43210-1086, USA
Abstract
Cryopreservation has become a very important tool for long-term stable storage of germplasm using minimum space and maintenance. The average survival rate of suspended cells of Acer mono was 54.4% when cells were cryoprotected with 1.2 M sucrose using a simple method. Survival rate of cells was lower with other cryoprotectants. Different survival rates were observed depending on methods used for cryopreservation of the cells. Vitrification showed the highest survival rate of 80.8% after 60 min of exposure to PVS2. After 40 min in PVS2, the survival rate of suspended cells decreased. There were no differences in RAPDs patterns between cryopreserved cells and the control. The results reported here clearly show that cryoperervation is a good method for long term conservation of tree species.
Key words: Acer mono, cryopreservation, cryotube, suspended cell, vitrification
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