Propagators routinely use a cold storage treatment for stem cuttings when propagating yew (Taxus spp. L.). Since storage conditions are a major factor in cutting quality and subsequent rooting, we examined the effect of storage temperature and duration on propagation success in four cultivars of Taxus x media Rehd. (Taxus baccata L. x T. cuspidata Sieb. & Zucc.) ('Brownii', 'Dark Green Spreader', 'Hicksii', and 'Wardii'). In addition, we attempted to quantify stress in relation to subsequent rooting using chlorophyll fluorescence (F_v/F_m). Cuttings were stored at either -2.5, 0, or 2.5°C for 0, 34, 70, or 107 days. There were no significant differences in F_v/F_m or adventitious rooting among the three temperatures tested. Thirty-four days of cold storage was beneficial, but longer storage durations (70 and 107 days) had negative effects on rooting success. Decreases in rooting percentages were found in all cultivars and 'Dark Green Spreader' appeared to be the least tolerant to long-term storage. Several positive correlations existed between F_v/F_m measurements of cuttings during storage and eventual rooting percentage. However, correlations lacked consistency. Consequently, chlorophyll fluorescence values, as measured by F_v/F_m ratios, are not consistent indicators of storage conditions for yew as they pertain to adventitious rooting.