In vitro somatic embryos from mature zygotic embryogenesis from Zizyphus jujuba Mill. have been investigated. When zygotic embryos were cultured on MS medium with varying concentration of 2,4-D, somatic embryos were formed from two varieties. Zizyphus jujuba var. inermis Rehd. showed better in vitro response in inducing somatic embryos than that of Zizyphus jujuba Mill. The optimum concentration for somatic embryogenesis from mature zygotic embyos was at 5 µM 2,4-D for Zizyphus jujuba var. inermis Rehd. and 40 µM 2,4-D for Zizyphus jujuba Mill. Repetitive secondary embryos from primary somatic embryos of Zizyphus jujuba var. inermis Rehd. were induced on MS medium containing 0.5 µM 2,4-D. Different stages of embyogenic structures, normal, and even abnormal, were observed on the induction medium. The germination and elongation of somatic embryos were effective on the MS medium without growth regulators or with 0.5 µM GA3, respectively. Somatic embryos of Zizyphus were developed to root and shoot.